DISTRIBUTION OF POLYMORPHIC GENOTYPES VARIANTS BSMI AND FOKI OF VITAMIN D RECEPTOR GENE IN PATIENTS WITH CARDIOVASCULAR DISEASE
Objective. To investigate the incidence of polymorphic markers Bsml (B/b) (rs1S44410) and Fokl (F/f) (rs2228S70) of the VDR gene in patients with cardiovascular diseases among the Grodno region population.
Materials and methods. 198 patients were examined, including 101 patients with arterial hypertension (AH) gr. ll and 97 patients with ischemic heart disease (lHD) with concomitant AH gr. ll. The blood serum vitamin D levels were determined using ELlSA. The Bsml polymorphism of the VDR gene was determined using the polymerase chain reaction (PCR), the Fokl polymorphism of the VDR gene was determined analyzing the polymorphism of the fragment lengths obtained during the PCR product restriction.
Results. Among the Grodno region patients with AH and lHD with concomitant AH, the least frequency was seen for the AA genotype — 1S% of the Bsml polymorphic marker and the ff genotype — 20% of the Fokl polymorphic marker of the VDR gene. The Ff genotype of the polymorphic marker Fokl of the VDR gene was identified most frequently— 46% compared to the other two genotypes. The genetic structure of the groups did not depend on the gender and the assessed genotypes incidence did not differ between men and women. The frequency of occurrence of different genotypes of the Bsml and Fokl polymorphic markers of the VDR gene did not differ in the groups of patients with D-hypovitaminosis and of those having blood vitamin D optimal level.